Reaction | Reacting groups | Substrates | Product | Seq description | DNA capping |
Group 1 - DNA 5'-PO4-2 oxygen Group 2 - ATP |
S: GGAAGCATAGCGAC-N70-AGCTGATCCTGATGG |
5',5'-AppDNA | N 70 |
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Buffer conditions |
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50 mM HEPES pH 7.0, 400 mM NaCl, 100 mM KCl, 10 mM MgCl2, 5 mM CaCl2, 1 mM MnCl2, and 50 µM CuCl2, 1mM ATP |
kcat/ kobs |
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kobs = 0.003 min-1 |
Catalytic region of the DNAzyme |
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GGCGGGAGAAGGGGGGTTGGGGTCTCGAGAACATGAGGATATGGCGTAAAAGTACTTATGAGTAGGGT |
Year of Publication | First Author | Laboratory | Title | PubMed ID | DOI | Reaction |
---|---|---|---|---|---|---|
2000 | Y Li | R R Breaker | Capping DNA with DNA. | 10715132 | 10.1021/bi992710r | DNA capping |