| Reaction | Reacting groups | Substrates | Product | Metal ion | Seq description | RNA cleavage |
Group 1 - 2'-OH Group 2 - vicinal phosphate |
X: F = fluorescein-dT, Q = DABCYL-dT S: GATGTGTCCGTGCFrAQGGTTCGA |
cleavage of single ribonucleotide phosphodiester |
Mn2+ |
N 70 |
|---|
| Buffer conditions |
|---|
| 400 mM NaCl, 100 mM KCl, 8.5 mM MgCl2, 5 mM MnCl2, 1.25 mM CdCl2, 0.25 mM NiCl2, citrate pH 5.0 |
| kcat/ kobs |
|---|
| kobs = 0.72 min-1 |
| Catalytic region of the DNAzyme |
|---|
| TGAATAGGGTCTCGGGCATAAATTACGGAAACGGTTTTAATTTTCTAGTGGAAAGGTCCGATAACGAGGT |
| Notes |
|---|
| Evolved from a population initially selected at pH 4.0. Also appeared in pH3 and pH4 pools as pH3DZ10 and pH4DZ2 |
| Year of Publication | First Author | Laboratory | Title | PubMed ID | DOI | Reaction |
|---|---|---|---|---|---|---|
| 2003 | Z Liu | Y Li | Assemblage of signaling DNA enzymes with intriguing metal-ion specificities and pH dependences. | 12812493 | 10.1021/ja035208+ | RNA cleavage |