| Reaction | Reacting groups | Substrates | Product | Metal ion | Linkage | Seq description | DNA ligation |
Group 1 - 3'-phosphorimidazolide Group 2 - 5'-OH |
L: AAGCATCTCAAGC R: GGAACACTATCCG |
activated substrate ligated to the ss DNA pool |
Zn2+ |
phosphodiester | N 116 |
|---|
| Buffer conditions |
|---|
| 30 mM HEPES pH 7.4, 600 mM KCl, 50 mM MgCl2, 1 mM ZnCl2 |
| Catalytic region of the DNAzyme |
|---|
| TTTGGTTTCGCAGTTGGTGTGTTCGTTCGCAGACCCTTTGGGTGAGATTGCTTTTGCGGCTTTGAGTGATCCTGCCTTGTGGTATTGTTGTGCATGTGATAGCTTGTTCTGCTCAT |
| Notes |
|---|
| R is the 5' pool's constant region, therefore connected to the N116 |
| Year of Publication | First Author | Laboratory | Title | PubMed ID | DOI | Reaction |
|---|---|---|---|---|---|---|
| 1995 | B Cuenoud | J W Szostak | A DNA metalloenzyme with DNA ligase activity. | 7791880 | 10.1038/375611a0 | DNA ligation |