| Reaction | Reacting groups | Substrates | Product | Metal ion | Linkage | Seq description | Covalent Modification of Amino Acid Side Chains |
Group 1 - 3'-OH Group 2 - 5'-triphosphate |
L: GGATAATACGXTTCACTGCG R: GGAAGAGAUGGCGACGG X: Tyr, Ala, Ser, rA, dA |
nucleopeptide linkage |
Mg2+ |
DNA-Tyr-DNA-RNA | N * |
|---|
| Buffer conditions |
|---|
| 50 mm CHES pH 9.0, 150 mM NaCl, 2 mM KCl, 40 mM MgCl2 |
| Catalytic region of the DNAzyme |
|---|
| GGTCAACAGTTTGGTTGGTTAAGTAGGGGGCCCCGCAGTGAGCAAGTTG |
| Notes |
|---|
| Pool N33-CGCAGTGAG-N7, the deoxyribozyme's sequence is anotated with the 9-nt linker between the two initially randomized regions |
| Year of Publication | First Author | Laboratory | Title | PubMed ID | DOI | Reaction |
|---|---|---|---|---|---|---|
| 2008 | P I Pradeepkumar | Scott K Silverman | DNA-catalyzed formation of nucleopeptide linkages. | 18214866 | 10.1002/anie.200703676 | Covalent Modification of Amino Acid Side Chains |