| Reaction | Reacting groups | Substrates | Product | Metal ion | Linkage | Seq description | Covalent Modification of Amino Acid Side Chains |
Group 1 - Tyr Group 2 - 5'-triphosphate |
L: GGATAATACGXTTCACTGCG R: GGAAGAGAUGGCGACGG X: Tyr |
nucleopeptide linkage |
Mn2+ Mg2+ |
Tyr-RNA | N * |
|---|
| Buffer conditions |
|---|
| 50 mm CHES pH 9.0, 150 mM NaCl, 2 mM KCl, 40 mM MgCl2 |
| Yield (%) | kcat/ kobs |
|---|---|
| 72 | kobs = 0.06 min-1 |
| Catalytic region of the DNAzyme |
|---|
| CACGACAGCTAGAAAGAGGTCTAAAAAGTACTCCGCAGTGAGCGACGCG |
| Notes |
|---|
| Pool N33-CGCAGTGAG-N7, the deoxyribozyme's sequence is anotated with the 9-nt linker between the two initially randomized regions |
| Year of Publication | First Author | Laboratory | Title | PubMed ID | DOI | Reaction |
|---|---|---|---|---|---|---|
| 2008 | P I Pradeepkumar | Scott K Silverman | DNA-catalyzed formation of nucleopeptide linkages. | 18214866 | 10.1002/anie.200703676 | Covalent Modification of Amino Acid Side Chains |