Reaction | Reacting groups | Substrates | Product | Metal ion | Linkage | Seq description | Covalent Modification of Amino Acid Side Chains |
Group 1 - Tyr Group 2 - 5'-triphosphate |
L: GGATAATACGACTCACTATX R: GGAAGAGAUGGCGACGG X: HEG-Cys-Tyr-Ala |
nucleopeptide linkage |
Mn2+ Mg2+ |
Tyr-RNA | N 30 |
---|
Buffer conditions |
---|
50 mM HEPES pH 7.5, 20 mM MnCl2, 40 mM MgCl2, 150 mM NaCl, 2 mM KCl |
Yield (%) | kcat/ kobs |
---|---|
45-55 | kobs = 0.15 h-1 |
Catalytic region of the DNAzyme |
---|
GCGCTGGGAGGCACATGCTGGGTTGCACCG |
Year of Publication | First Author | Laboratory | Title | PubMed ID | DOI | Reaction |
---|---|---|---|---|---|---|
2012 | T E Velez | S K Silverman | Systematic evaluation of the dependence of deoxyribozyme catalysis on random region length. | 23088677 | 10.1021/co300111f | Covalent Modification of Amino Acid Side Chains |