| Reaction | Reacting groups | Substrates | Product | Metal ion | Linkage | Seq description | RNA cleavage |
Group 1 - 2'-OH of R8 Group 2 - vicinal phosphate |
S: NNNNNNNRYNNNNNN |
specific cleavage at desired position |
Mg2+ |
RNA phosphodiester | N 50 |
|---|
| Buffer conditions |
|---|
| 2 mM MgCl2, 150 mM NaCl, and 50 mM Tris⋅HCl pH 7.5 |
| kcat/ kobs |
|---|
| kcat = 3.4 min-1 |
| Catalytic region of the DNAzyme |
|---|
| RGGCTAGCTACAACGA |
| Notes |
|---|
| Obtained after re-selection |
| 3D structures |
|---|
| 1BR3 |
| 1EGK |
| Year of Publication | First Author | Laboratory | Title | PubMed ID | DOI | Reaction |
|---|---|---|---|---|---|---|
| 1997 | S W Santoro | G F Joyce | A general purpose RNA-cleaving DNA enzyme. | 9113977 | 10.1073/pnas.94.9.4262 | RNA cleavage |
| Year of Publication | First Author | Laboratory | Title | PubMed ID | DOI | Reaction |
|---|---|---|---|---|---|---|
| 2003 | M J Cairns | L‐Q Sun | Optimisation of the 10-23 DNAzyme-substrate pairing interactions enhanced RNA cleavage activity at purine-cytosine target sites. | 12771215 | 10.1093/nar/gkg378 |
RNA cleavage |