| Reaction | Reacting groups | Substrates | Product | Metal ion | Linkage | Seq description | RNA ligation |
Group 1 - 2',3'-cyclic phosphate Group 2 - 5'-OH |
L: UAAUACGACUCACUAUA R: GGAAGAGAUGGCGACGG |
non-native RNA |
Mg2+ |
2',5' | N 40 |
|---|
| Buffer conditions |
|---|
| 50 mM CHES pH 9.0, 40 mM MgCl2 |
| Yield (%) | kcat/ kobs |
|---|---|
| 25-35 | kobs = 0.2-0.3 h-1 |
| Catalytic region of the DNAzyme |
|---|
| GGCGTTAAGGATTGGCGGAAACGGGTGGATCGCGGACC |
| Year of Publication | First Author | Laboratory | Title | PubMed ID | DOI | Reaction |
|---|---|---|---|---|---|---|
| 2005 | D R Semlow | S K Silverman | Parallel selections in vitro reveal a preference for 2'-5' RNA ligation upon deoxyribozyme-mediated opening of a 2',3'-cyclic phosphate. | 16007488 | 10.1007/s00239-004-0326-y | RNA ligation |