Reaction | Reacting groups | Substrates | Product | Metal ion | Linkage | Seq description | RNA ligation |
Group 1 - internal 2'-OH Group 2 - 5'-triphosphate |
L: GGAUAAUACGUCUCAC R: GGAAGAGAUGGCGACGG |
branched RNA |
Mg2+ |
A8 | N * |
---|
Buffer conditions |
---|
50 mM CHES pH 9.0, 150 mM NaCl, 2 mM KCl, 40 mM MgCl2 |
Yield (%) | kcat/ kobs |
---|---|
>80 | kobs = 0.27 min-1 |
Catalytic region of the DNAzyme |
---|
CCTATGGCCCAGGGCGTGAGGGTGCGGTTCC |
Notes |
---|
Pool N15-GTGAG-N7 |
Year of Publication | First Author | Laboratory | Title | PubMed ID | DOI | Reaction |
---|---|---|---|---|---|---|
2006 | E Zelin | Scott K Silverman | Adenosine is inherently favored as the branch-site RNA nucleotide in a structural context that resembles natural RNA splicing. | 16503631 | 10.1021/bi052499l | RNA ligation |