| Reaction | Reacting groups | Substrates | Product | Metal ion | Linkage | Seq description | RNA ligation |
Group 1 - 2',3'-diol Group 2 - 5'-triphosphate |
L: d(GAACTGACGAACTGATGCTCAC)-r(UAUA) R: GAGACCGUAAUGAGUAG |
non-native RNA |
Mg2+ |
2',5' | N * |
|---|
| Buffer conditions |
|---|
| 25 mM MgCl2, 25 mM EPPS pH 8.5 |
| Catalytic region of the DNAzyme |
|---|
| CTATGCATTATCCTTGACTGTTAGATCGAGCAGTTGCAACGGGTTGATTATAGTGAG |
| Notes |
|---|
| Evolved from R3C ribozyme N51, binding arms anotated together with the catalytic core |
| Year of Publication | First Author | Laboratory | Title | PubMed ID | DOI | Reaction |
|---|---|---|---|---|---|---|
| 2006 | N Paul | G F Joyce | Conversion of a ribozyme to a deoxyribozyme through in vitro evolution. | 16638538 | 10.1016/j.chembiol.2006.01.007 | RNA ligation |