| Reaction | Reacting groups | Substrates | Product | Metal ion | Linkage | Seq description | RNA ligation |
Group 1 - internal 2'-OH Group 2 - 5'-triphosphate |
L: GGAAGUCUCAUGUACUAUCG R: GAAUGUUCUAGCGCGGA |
branched RNA |
Mn2+ |
U16 | N 20 |
|---|
| Buffer conditions |
|---|
| 50 mM HEPES pH 7.5, 150 mM NaCl, 2 mM KCl, 20 mM MnCl2 |
| Yield (%) | kcat/ kobs |
|---|---|
| 90 | kobs = 0.67 h-1 |
| Catalytic region of the DNAzyme |
|---|
| GGCACTCAGAGCGCACGGCG |
| Year of Publication | First Author | Laboratory | Title | PubMed ID | DOI | Reaction |
|---|---|---|---|---|---|---|
| 2005 | E D Pratico | S K Silverman | A deoxyribozyme that synthesizes 2',5'-branched RNA with any branch-site nucleotide. | 15967808 | 10.1093/nar/gki656 | RNA ligation |