| Reaction | Reacting groups | Substrates | Product | Metal ion | Linkage | Seq description | RNA ligation |
Group 1 - internal 2'-OH Group 2 - 5'-triphosphate |
L: UAAUACGACUCACUAUA R: GGAAGAGAUGGCGACGG |
branched RNA |
Mg2+ |
A8 | N 37 |
|---|
| Buffer conditions |
|---|
| 50 mM CHES pH 9.0, 150 mM NaCl, 2 mM KCl, 40 mM MgCl2 |
| Yield (%) | kcat/ kobs |
|---|---|
| >90 | kobs = 0.5 min-1 |
| Catalytic region of the DNAzyme |
|---|
| CAGTGCAGGGCGTGAGGGCTCGGTTCCCGTATTATCT |
| Notes |
|---|
| LARIAT FORMATION |
| Year of Publication | First Author | Laboratory | Title | PubMed ID | DOI | Reaction |
|---|---|---|---|---|---|---|
| 2004 | R L Coppins | S K Silverman | A DNA enzyme that mimics the first step of RNA splicing. | 14758353 | 10.1038/nsmb727 | RNA ligation |