| Reaction | Reacting groups | Substrates | Product | Metal ion | Linkage | Seq description | RNA ligation |
Group 1 - 2',3'-cyclic phosphate Group 2 - 5'-OH |
L: UAAUACGACUCACUAUA R: GGAAGAGAUGGCGACGG |
unnatural |
Zn2+ |
* | N 40 |
|---|
| Buffer conditions |
|---|
| 70 mM Tris pH 7.9, 150 mM NaCl, 2 mM KCl, 1 mM ZnCl2 |
| Yield (%) | kcat/ kobs |
|---|---|
| 15 | kobs = 0.018 min-1 |
| Catalytic region of the DNAzyme |
|---|
| TGCACGGGGGGTGAGGTTTCCGCCGTTGAAACGTAGCACG |
| Notes |
|---|
| 3'-2' or 2'-2' linear junctions (whichever is not formed by 6J12) |
| Year of Publication | First Author | Laboratory | Title | PubMed ID | DOI | Reaction |
|---|---|---|---|---|---|---|
| 2005 | K A Hoadley | S K Silverman | Zn2+-dependent deoxyribozymes that form natural and unnatural RNA linkages. | 15966746 | 10.1021/bi050146g | RNA ligation |