| Reaction | Reacting groups | Substrates | Product | Metal ion | Linkage | Seq description | RNA ligation |
Group 1 - 2',3 '- diol Group 2 - 5'-triphosphate |
L: GGAAGUCUCAUGUACUA R: GAUGUUCUAGCGCCGGA |
native RNA |
Zn2+ |
3',5' | N 40 |
|---|
| Buffer conditions |
|---|
| 70 mM Tris pH 7.5, 150 mM NaCl, 2 mM KCl, 1 mM ZnCl2 |
| Yield (%) | kcat/ kobs |
|---|---|
| 40-50 | kobs = 0.02 (min-1) at pH 7.5 |
| Catalytic region of the DNAzyme |
|---|
| GTAGGGTTGGTAGACCAGGTTGAGCCGGCGTCCTTGTTTA |
| Year of Publication | First Author | Laboratory | Title | PubMed ID | DOI | Reaction |
|---|---|---|---|---|---|---|
| 2005 | W E Purtha | S K Silverman | General deoxyribozyme-catalyzed synthesis of native 3'-5' RNA linkages. | 16173722 | 10.1021/ja0533702 | RNA ligation |