| Reaction | Reacting groups | Substrates | Product | Metal ion | Linkage | Seq description | RNA ligation |
Group 1 - internal 2'-OH Group 2 - 5'-triphosphate |
L: UAAUACGACUCACUAUX R: GGAAGAGAUGGCGACGG X: A, C, dA, dC |
branched RNA |
Mn2+ Mg2+ |
A15 | N 40 |
|---|
| Buffer conditions |
|---|
| 50 mM HEPES pH 7.5, 20 mM MnCl2 |
| Yield (%) | kcat/ kobs |
|---|---|
| >85 | kobs = 0.27 (min-1) at pH 7.5 |
| Catalytic region of the DNAzyme |
|---|
| AATGAGGCTTGGCAGGGATTTAGTATTTTAACACTCCCGG |
| Notes |
|---|
| LARIAT FORMATION |
| Year of Publication | First Author | Laboratory | Title | PubMed ID | DOI | Reaction |
|---|---|---|---|---|---|---|
| 2003 | Y Wang | S K Silverman | Deoxyribozymes that synthesize branched and lariat RNA. | 12783536 | 10.1021/ja035150z | RNA ligation |
| Year of Publication | First Author | Laboratory | Title | PubMed ID | DOI | Reaction |
|---|---|---|---|---|---|---|
| 2003 | Y Wang | S K Silverman | Characterization of deoxyribozymes that synthesize branched RNA. | 14690435 | 10.1021/bi0355847 |
RNA ligation |
| 2004 | R L Coppins | S K Silverman | A DNA enzyme that mimics the first step of RNA splicing. | 14758353 | 10.1038/nsmb727 |
RNA ligation |
| 2013 | F Javadi-Zarnaghi | Lanthanide cofactors accelerate DNA-catalyzed synthesis of branched RNA. | 23895365 | 10.1021/ja406162z |
RNA ligation |