| Reaction | Reacting groups | Substrates | Product | Metal ion | Linkage | Seq description | RNA ligation |
Group 1 - 2',3'-cyclic phosphate Group 2 - 5'-OH |
L: UAAUACGACUCACUAUA R: GGAAGAGAUGGCGACGG |
non-native RNA |
Mg2+ |
2',5' | N 40 |
|---|
| Buffer conditions |
|---|
| 50 mM HEPES pH 7.5, 150 mM NaCl, 2 mM KCl, 40 mM MgCl2 |
| Yield (%) | kcat/ kobs |
|---|---|
| 40-60 | kobs = 0.08 (h-1) at pH 7.5, kobs = 0.023 (min-1) at pH 9.0 |
| Catalytic region of the DNAzyme |
|---|
| TGCGTTCTGATGAATTGCACATAGCTTATAGTTCCTTGCT |
| Year of Publication | First Author | Laboratory | Title | PubMed ID | DOI | Reaction |
|---|---|---|---|---|---|---|
| 2003 | A Flynn-Charlebois | S K Silverman | Deoxyribozymes with 2'-5' RNA ligase activity. | 12603132 | 10.1021/ja028774y | RNA ligation |
| Year of Publication | First Author | Laboratory | Title | PubMed ID | DOI | Reaction |
|---|---|---|---|---|---|---|
| 2004 | T K Prior | S K Silverman | Structure-function correlations derived from faster variants of a RNA ligase deoxyribozyme. | 14960718 | 10.1093/nar/gkh263 |
RNA ligation |