Reaction | Reacting groups | Substrates | Product | Metal ion | Linkage | Seq description | RNA ligation |
Group 1 - internal 2'-OH Group 2 - 5'-triphosphate |
L: GGAAGUCUCAUGUACUAACA R: GUAUGUUCUAGCGCGGA |
branched RNA |
Mn2+ |
C19 | N 40 |
---|
Buffer conditions |
---|
50 mM HEPES pH 7.5, 150 mM NaCl, 2 mM KCl, 20 mM MnCl2 |
Catalytic region of the DNAzyme |
---|
GCAGTGCTAATGAGGTATCGCAAATAAGTGCGCAGCCGAA |
Year of Publication | First Author | Laboratory | Title | PubMed ID | DOI | Reaction |
---|---|---|---|---|---|---|
2005 | Y Wang | S K Silverman | Directing the outcome of deoxyribozyme selections to favor native 3'-5' RNA ligation. | 15723545 | 10.1021/bi0478291 | RNA ligation |