| Reaction | Reacting groups | Substrates | Product | Metal ion | Linkage | Seq description | RNA cleavage |
Group 1 - 3'-OH of rA Group 2 - vicinal phosphate |
S: GTCACGAGTCACTATrAGGAAGATGGCGAAA |
specific cleavage at desired position |
Ce3+ |
2'-5'-RNA linkage | N 50 |
|---|
| Buffer conditions |
|---|
| 50 mM MES pH 6.0, 50 mM KCl, 10 μM Ce3+ |
| kcat/ kobs |
|---|
| kobs = 0.16 min-1 |
| Catalytic region of the DNAzyme |
|---|
| TTTCGCCATCTTTAGGAATATCTATTCCACGGCTCACGAAATAGTGACTCGTGAC |
| Year of Publication | First Author | Laboratory | Title | PubMed ID | DOI | Reaction |
|---|---|---|---|---|---|---|
| 2016 | W Zhou | J Liu | An Efficient Lanthanide-Dependent DNAzyme Cleaving 2'-5'-Linked RNA. | 26957420 | 10.1002/cbic.201500690 | RNA cleavage |