| Reaction | Reacting groups | Substrates | Product | Metal ion | Linkage | Seq description | RNA cleavage |
Group 1 - 2'-OH of rU Group 2 - vicinal phosphate |
S: GAGAGAGATrUCGTGCGTTAC |
cleavage at UC junction |
Mn2+ |
RNA phosphodiester | N 20 |
|---|
| Buffer conditions |
|---|
| 100 mM KCl, 400 mM NaCl, 7.5 mM MgCl2 , 7.5 mM MnCl2, 50 mM HEPES pH 7.0 |
| Yield (%) | kcat/ kobs |
|---|---|
| 85 | kobs = 0.009 min-1 |
| Catalytic region of the DNAzyme |
|---|
| CGGGGTTTGGGATGAAGGGG |
| Year of Publication | First Author | Laboratory | Title | PubMed ID | DOI | Reaction |
|---|---|---|---|---|---|---|
| 2008 | K Schlosser | Y Li | In vitro selection of small RNA-cleaving deoxyribozymes that cleave pyrimidine-pyrimidine junctions. | 18644842 | 10.1093/nar/gkn396 | RNA cleavage |