| Reaction | Reacting groups | Substrates | Product | Metal ion | Linkage | Seq description | RNA cleavage |
Group 1 - 2'-OH of rG Group 2 - vicinal phosphate |
S: GGCGUGCCCGUCUGUUGGC |
* |
M2+-independent |
RNA phosphodiester | N 40 |
|---|
| Buffer conditions |
|---|
| 50 mM cacodylate pH 7.45, 200 mM NaCl, 1 mM EDTA |
| kcat/ kobs |
|---|
| kcat = 1.06 min-1 |
| Catalytic region of the DNAzyme |
|---|
| TTACAGTGGTATCGATTGGGACGTGTGGAGCGTCAGGATGGTACA |
| Notes |
|---|
| Reselected from 7-38. Engineered to act in trans. Cleavage site G10. |
| Year of Publication | First Author | Laboratory | Title | PubMed ID | DOI | Reaction |
|---|---|---|---|---|---|---|
| 2018 | Y Wang | D M Perrin | A densely modified M2+-independent DNAzyme that cleaves RNA efficiently with multiple catalytic turnover | None | 10.1039/C7SC04491G | RNA cleavage |