| Reaction | Reacting groups | Substrates | Product | Metal ion | Linkage | Seq description | RNA cleavage |
Group 1 - 2'-OH of rA Group 2 - vicinal phosphate |
S: ACTCACTATrAGGAAGAGATG |
specific cleavage at desired position |
Zn2+ |
RNA phosphodiester | N * |
|---|
| Buffer conditions |
|---|
| ZnCl2, 50 mM HEPES pH 7.0 |
| kcat/ kobs |
|---|
| kmax = 1.35 min-1 |
| Catalytic region of the DNAzyme |
|---|
| CATCTCTTCTCCGAGCCGGTCGAAATAGTGAGT |
| Notes |
|---|
| Trans-cleaving format derived from class I reselection. Similar to 8-17. |
| Year of Publication | First Author | Laboratory | Title | PubMed ID | DOI | Reaction |
|---|---|---|---|---|---|---|
| 2000 | J Li | Y Lu | In vitro selection and characterization of a highly efficient Zn(II)-dependent RNA-cleaving deoxyribozyme. | 10606646 | 10.1093/nar/28.2.481 | RNA cleavage |
| Year of Publication | First Author | Laboratory | Title | PubMed ID | DOI | Reaction |
|---|---|---|---|---|---|---|
| 2000 | J Li | Y Lu | A Highly Sensitive and Selective Catalytic DNA Biosensor for Lead Ions | None | 10.1021/ja0021316 |
RNA cleavage |