Reaction | Reacting groups | Substrates | Product | Metal ion | Linkage | Seq description | RNA cleavage |
Group 1 - 2'-OH of rA Group 2 - vicinal phosphate |
S: cleavage in cis |
specific cleavage at desired position |
Ce3+ |
RNA phosphodiester | N 50 |
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Buffer conditions |
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50 mM MES pH 6.0, 25 mM NaCl, Ce(IV) |
Catalytic region of the DNAzyme |
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CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATCTTTACGACATCCGGGGCATGAACCACGATGGCCATATATAACGAATGGGTTATAGTGACGGTAAGCTTGGCAC |
Year of Publication | First Author | Laboratory | Title | PubMed ID | DOI | Reaction |
---|---|---|---|---|---|---|
2014 | P-J J Huang | J Liu | Ultrasensitive DNAzyme beacon for lanthanides and metal speciation. | 24383540 | 10.1021/ac403762s | RNA cleavage |