| Reaction | Reacting groups | Substrates | Product | Metal ion | Linkage | Seq description | RNA cleavage |
Group 1 - 2'-OH of rA Group 2 - vicinal phosphate |
S: cleavage in cis |
specific cleavage at desired position |
Na+ |
RNA phosphodiester | N 50 |
|---|
| Buffer conditions |
|---|
| 10 µM of Co(NH3)6Cl3, 50 mM MES pH 6.0, 25 mM NaCl, 1 mM EDTA |
| kcat/ kobs |
|---|
| kobs = 0.11 min-1 |
| Catalytic region of the DNAzyme |
|---|
| GGAGCCATAGGTCAAAGGTGGGTGTGGTCGTATCATATCGACCAGCAWT |
| Notes |
|---|
| Very similar to NaA43 and to Ce13d |
| Year of Publication | First Author | Laboratory | Title | PubMed ID | DOI | Reaction |
|---|---|---|---|---|---|---|
| 2019 | L Ma | J Liu | An in Vitro-Selected DNAzyme Mutant Highly Specific for Na under Slightly Acidic Conditions. | 29989277 | 10.1002/cbic.201800322 | RNA cleavage |
| Year of Publication | First Author | Laboratory | Title | PubMed ID | DOI | Reaction |
|---|---|---|---|---|---|---|
| 2019 | L Ma | J Liu | From general base to general acid catalysis in a sodium-specific DNAzyme by a guanine-to-adenine mutation | None | 10.1093/nar/gkz578 |
RNA cleavage |
| 2015 | S-F Torabi | Y Lu | In vitro selection of a sodium-specific DNAzyme and its application in intracellular sensing | None | 10.1073/pnas.1420361112 |
RNA cleavage |