| Reaction | Reacting groups | Substrates | Product | Metal ion | Linkage | Seq description | RNA ligation |
Group 1 - 3 '- OH Group 2 - 5'-adenylated RNA |
L: GGCGAACUCUUCGA R: GAGCUGAUCCUGAGAA |
native RNA |
Mn2+ |
3',5' | N 40 |
|---|
| Buffer conditions |
|---|
| 50 mM HEPES pH 7.5, 2 mM KCl, 150 mM NaCl, MgCl2, 20 mM MnCl2 |
| Yield (%) | kcat/ kobs |
|---|---|
| 80-90 | kobs = 0.0219 min-1 |
| Catalytic region of the DNAzyme |
|---|
| GTACGGACGTCAGTCACGAGGCAGCTGCTTTGCCAAACTG |
| Year of Publication | First Author | Laboratory | Title | PubMed ID | DOI | Reaction |
|---|---|---|---|---|---|---|
| 2020 | C P M Scheitl | C Höbartner | New Deoxyribozymes for the Native Ligation of RNA. | 32796587 | 10.3390/molecules25163650 | RNA ligation |