| Reaction | Reacting groups | Substrates | Product | Metal ion | Linkage | Seq description | RNA cleavage |
Group 1 - 2'-OH of rA Group 2 - vicinal phosphate |
S: cleavage in cis |
specific cleavage at desired position |
Zn2+ |
RNA phosphodiester | N 40 |
|---|
| Buffer conditions |
|---|
| 500 mM NaCl, 50 mM HEPES pH 7.0, ZnCl2 0.1 µM |
| Yield (%) | kcat/ kobs |
|---|---|
| 53 | kobs = 0.929 x 10-2 min-1 |
| Catalytic region of the DNAzyme |
|---|
| CATCTCGGGACCGTAGACCACGTGCGGAAGGTGGTCACGGCTTGCTGTACG |
| Notes |
|---|
| The annotated sequence contains 3' and 5' conserved regions in addition to the N<sub>40</sub> region. |
| Year of Publication | First Author | Laboratory | Title | PubMed ID | DOI | Reaction |
|---|---|---|---|---|---|---|
| 2005 | K E Nelson | Y Lu | In vitro selection of high temperature Zn(2+)-dependent DNAzymes. | 16096680 | 10.1007/s00239-004-0374-3 | RNA cleavage |