| Reaction | Reacting groups | Substrates | Product | Metal ion | Linkage | Seq description | RNA cleavage |
Group 1 - 2'-OH of rA Group 2 - vicinal phosphate |
X: Fluorescein (F)- and a DABCYL (Q)-modified deoxyribothymidine. S: GATGTGTCCGTGCFrAQGGTTCGATTCTTGTGACT |
specific cleavage at desired position |
Co2+ |
RNA phosphodiester | N * |
|---|
| Buffer conditions |
|---|
| 10 mM CoCl2, 5 mM MgCl2, 50 mM HEPES pH 6.8 |
| kcat/ kobs |
|---|
| kcat = 7.2 min-1 |
| Catalytic region of the DNAzyme |
|---|
| AAGAATCGTTGTCATTGGCACACGGAGGTTTACTGAGTGGTAACCACGTTGCATGGAA |
| Notes |
|---|
| Trans-cleaving version of DEC22-18. |
| Year of Publication | First Author | Laboratory | Title | PubMed ID | DOI | Reaction |
|---|---|---|---|---|---|---|
| 2003 | S H J Mei | Y Li | An efficient RNA-cleaving DNA enzyme that synchronizes catalysis with fluorescence signaling. | 12517153 | 10.1021/ja0281232 | RNA cleavage |