| Reaction | Reacting groups | Substrates | Product | Metal ion | Linkage | Seq description | RNA cleavage |
Group 1 - 2'-OH of rA Group 2 - vicinal phosphate |
X: Fluorescein (F)- and a DABCYL (Q)-modified deoxyribothymidine. S: cleavage in cis |
specific cleavage at desired position |
Co2+ |
RNA phosphodiester | N 43 |
|---|
| Buffer conditions |
|---|
| 50 mM HEPES pH 6.8, 400 mM NaCl, 200 mM KCl, 7.5 mM MgCl2, 5 mM MnCl2, 1.25 mM CdCl2, 1 mM CoCl2, 0.25 mM NiCl2 |
| kcat/ kobs |
|---|
| kobs = 1 min-1 |
| Catalytic region of the DNAzyme |
|---|
| GATGTGTCCGTGCFAQGGTTCGATTCTTGATCGTTGTCATTGGCACACGGAGGTTTACTGAGTGGTAACCACGTTGCATGGAATTCGGTAAGCTTGGCACCCGCATCGT |
| Notes |
|---|
| A deletion mutant of DEC22-18 termed DEC22-18A works optimally in the absence of monovalent ions and displays a k<sub>obs</sub> = 10 min<sup>-1</sup>. |
| Year of Publication | First Author | Laboratory | Title | PubMed ID | DOI | Reaction |
|---|---|---|---|---|---|---|
| 2003 | S H J Mei | Y Li | An efficient RNA-cleaving DNA enzyme that synchronizes catalysis with fluorescence signaling. | 12517153 | 10.1021/ja0281232 | RNA cleavage |