| Reaction | Reacting groups | Substrates | Product | Metal ion | Linkage | Seq description | RNA cleavage |
Group 1 - 2'-OH of rA Group 2 - vicinal phosphate |
S: * |
specific cleavage at desired position |
Na+ |
RNA phosphodiester | N 50 |
|---|
| Buffer conditions |
|---|
| 50 mM Bis-Tris pH 7.0, 1 mM EDTA, 10 mM sodium citrate, 370 mM NaCl |
| Catalytic region of the DNAzyme |
|---|
| GTTCAGACTAATCATCACGTATAGGAAGTACCGCATGGTACTAGGTCAAAGGTTTGGTG—AGGGGACGCCAAGAGTCCCCGCGGTTATGTGATGTGAGCCTACGATGAGAC |
| Notes |
|---|
| This is the cis-cleaving version of the DNAzyme. Sequences have been annotated as reported. The cleavage site rA is in position 23 in the reported sequence. |
| Year of Publication | First Author | Laboratory | Title | PubMed ID | DOI | Reaction |
|---|---|---|---|---|---|---|
| 2015 | S-F Torabi | Y Lu | In vitro selection of a sodium-specific DNAzyme and its application in intracellular sensing | None | 10.1073/pnas.1420361112 | RNA cleavage |