| Reaction | Reacting groups | Substrates | Product | Metal ion | Linkage | Seq description | Tyrosine azido‐adenylylation |
Group 1 - Tyrosine's hydroxyl Group 2 - 2′‐Az‐dATP |
L: DNA-anchored CLQTYPRT octapeptide R: 2′‐Az‐dATP |
Azido-adenylated peptide |
Mn2+ Mg2+ Zn2+ |
phosphodiester | N 40 |
|---|
| Buffer conditions |
|---|
| 70 mm HEPES pH 7.5, 1 mm ZnCl2, 20 mm MnCl2, 40 mm MgCl2, 150 mm NaCl |
| Yield (%) | kcat/ kobs |
|---|---|
| 70-80 | kobs = 0.14 h-1 |
| Catalytic region of the DNAzyme |
|---|
| GCACATACCGAATCAGAGCGGTGGCCAGGACATGAGATAA |
| Notes |
|---|
| Natural ATP is generally tolerated well in place of 2′‐Az‐dATP. |
| Year of Publication | First Author | Laboratory | Title | PubMed ID | DOI | Reaction |
|---|---|---|---|---|---|---|
| 2016 | P Wang | S K Silverman | DNA-Catalyzed Introduction of Azide at Tyrosine for Peptide Modification. | 27391404 | 10.1002/anie.201604364 | Tyrosine azido‐adenylylation |