| Reaction | Reacting groups | Substrates | Metal ion | Linkage | Seq description | RNA cleavage |
Group 1 - 2'-OH Group 2 - vicinal phosphate |
X: riboG S: AAAGTCTCATGTACTTATATXTTCTAGCGCgga |
Mn2+ Mg2+ Zn2+ |
RNA phosphodiester | N 40 |
|---|
| Buffer conditions |
|---|
| 70 mM HEPES pH 7.5, 1 mM ZnCl2, 20 mM MnCl2, 40 mM MgCl2, 150 mM NaCl |
| Yield (%) | kcat/ kobs |
|---|---|
| 80-90 | kobs = 1,38 h-1 |
| Catalytic region of the DNAzyme |
|---|
| CGATAGATAACGGGGTGGATTTGCAACCGCAGTACATATA |
| Notes |
|---|
| Partially randomized catalytic region based on 10MD5 sequence |
| Year of Publication | First Author | Laboratory | Title | PubMed ID | DOI | Reaction |
|---|---|---|---|---|---|---|
| 2013 | D J Parker | S K Silverman | DNA catalysis of a normally disfavored RNA hydrolysis reaction. | 23697866 | 10.1021/ja4032488 | RNA cleavage |
| Year of Publication | First Author | Laboratory | Title | PubMed ID | DOI | Reaction |
|---|---|---|---|---|---|---|
| 2009 | M Chandra | S K Silverman | DNA-catalyzed sequence-specific hydrolysis of DNA | None | 10.1038/nchembio.201 |
DNA cleavage |