| Reaction | Reacting groups | Substrates | Metal ion | Linkage | Seq description | Covalent Modification of Amino Acid Side Chains |
Group 1 - aliphatic amino group Group 2 - 5′-phosphorimidazolide |
L: DNA-C3-NH2 R: 5′-phosphorimidazolide-activated DNA oligonucleotide |
Mn2+ |
phosphoramidate (P–N) linkage | N 40 |
|---|
| Buffer conditions |
|---|
| 70 mM HEPES pH 7.5 with 40 mM MgCl2, 20 mM MnCl2, 1 mM ZnCl2, 150 mM NaCl |
| Yield (%) | kcat/ kobs |
|---|---|
| 80-90 | kobs = 0.37 h-1 |
| Catalytic region of the DNAzyme |
|---|
| CAAGGGGAACGAGGTTGTGAGAAGTAAGGGCGTGAGTAGC |
| Year of Publication | First Author | Laboratory | Title | PubMed ID | DOI | Reaction |
|---|---|---|---|---|---|---|
| 2014 | B M Brandsen | S K Silverman | DNA-catalyzed lysine side chain modification. | 24981820 | 10.1002/anie.201404622 | Covalent Modification of Amino Acid Side Chains |