under construction.......

References:

1. Yu B, Yang Z, Li J, Minakhina S, Yang M, Padgett RW, Steward R, Chen X. "Methylation as a crucial step in plant microRNA biogenesis." Science. 2005 Feb 11;307(5711):932-5.

2. Tkaczuk KL, Obarska A, Bujnicki JM., "Molecular phylogenetics and comparative modeling of HEN1, a methyltransferase involved in plant microRNA biogenesis", BMC Evol Biol. 2006 Jan 24;6:6.

3. Huang Y, Ji L, Huang Q, Vassylyev DG, Chen X, Ma JB. "Structural insights into mechanisms of the small RNA methyltransferase HEN1", Nature. 2009 Oct 8;461(7265):823-7

4. Mui Chan C, Zhou C, Brunzelle JS, Huang RH. "Structural and biochemical insights into 2'-O-methylation at the 3'-terminal nucleotide of RNA by Hen1", Proc Natl Acad Sci U S A. 2009 Oct 20;106(42):17699-704.

Download structures:

A.thaliana HEN1 model (catalytic domain only)

A. thaliana HEN1 structure (full-length protein in complex with RNA)

Read our manuscript:

Download PDF

Gallery:

Comparison of structures of our model and the native structure (PDB code:3htx). Structures are colored according to the sequence index (N-terminus – blue, C-terminus – red). We correctly predicted that HEN1 catalytic domain exhibits the RFM fold The model has very good quality in the catalytic region.

Superposition of the HEN1 catalytic domain model (green) and native structure (blue).